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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
25/04/2018 |
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Actualizado : |
25/04/2018 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GALLINO, J.P.; RUIBAL, C.; CASARETTO, E.; FLEITAS, A.L.; BONNECARRERE, V.; BORSANI, O.; VIDAL, S. |
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Afiliación : |
JUAN P. GALLINO, Universidad de la República (UdelaR)/ Facultad de Ciencias; CECILIA RUIBAL, Universidad de la República (UdelaR)/ Facultad de Ciencias; ESTEBAN CASARETTO, Universidad de la República (UdelaR)/ Facultad de Agronomía; ANDREA L. FLEITAS, Universidad de la República (UdelaR)/ Facultad de Ciencias; MARIA VICTORIA BONNECARRERE MARTINEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; OMAR BORSANI, Universidad de la República (UdelaR)/ Facultad de Agronomía; SABINA VIDAL, Universidad de la República (UdelaR)/ Facultad de Ciencias. |
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Título : |
A dehydration-induced eukaryotic translation initiation factor iso4G identified in a slow wilting soybean cultivar enhances abiotic stress tolerance in Arabidopsis. |
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Fecha de publicación : |
2018 |
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Fuente / Imprenta : |
Frontiers in Plant Science, 2018, v.9, Article number 262. (2 March 2018). OPEN ACCESS |
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DOI : |
10.3389/fpls.2018.00262 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received: 22 December 2017; Accepted: 14 February 2018; Published: 02 March 2018. |
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Contenido : |
ABSTRACT.
Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (GmeIFiso4G-1a). GmeIFiso4G-1a is one of four members of this protein family in soybean, all of them sharing high sequence identity with each other. In silico analysis of GmeIFiso4G-1 promoter sequences suggested a possible functional specialization between distinct family members, which can attain differences at the transcriptional level. Conditional overexpression of GmeIFiso4G-1a in Arabidopsis conferred the transgenic plants increased tolerance to osmotic, salt, drought and low temperature stress, providing a strong experimental evidence for a direct association between a protein of this class and general abiotic stress tolerance mechanisms. Moreover, the results of this work reinforce the importance of the control of protein synthesis as a central mechanism of stress adaptation and opens up for new strategies for improving crop performance under stress.
© 2018 Gallino, Ruibal, Casaretto, Fleitas, Bonnecarrère, Borsani and Vidal. MenosABSTRACT.
Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (Gm... Presentar Todo |
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Palabras claves : |
ABIOTIC STRESS; ARABIDOPSIS; DROUGHT; EIFiso4G; SOYBEAN CROP; TRANSLATION INITIATION. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/9385/1/Frontiers-in-Plant-Science.-2018.fpls-09-00262.pdf
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Marc : |
LEADER 03453naa a2200289 a 4500
001 1058480
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008 2018 bl uuuu u00u1 u #d
024 7 $a10.3389/fpls.2018.00262$2DOI
100 1 $aGALLINO, J.P.
245 $aA dehydration-induced eukaryotic translation initiation factor iso4G identified in a slow wilting soybean cultivar enhances abiotic stress tolerance in Arabidopsis.$h[electronic resource]
260 $c2018
500 $aArticle history: Received: 22 December 2017; Accepted: 14 February 2018; Published: 02 March 2018.
520 $aABSTRACT. Water is usually the main limiting factor for soybean productivity worldwide and yet advances in genetic improvement for drought resistance in this crop are still limited. In the present study, we investigated the physiological and molecular responses to drought in two soybean contrasting genotypes, a slow wilting N7001 and a drought sensitive TJS2049 cultivars. Measurements of stomatal conductance, carbon isotope ratios and accumulated dry matter showed that N7001 responds to drought by employing mechanisms resulting in a more efficient water use than TJS2049. To provide an insight into the molecular mechanisms that these cultivars employ to deal with water stress, their early and late transcriptional responses to drought were analyzed by suppression subtractive hybridization. A number of differentially regulated genes from N7001 were identified and their expression pattern was compared between in this genotype and TJS2049. Overall, the data set indicated that N7001 responds to drought earlier than TJ2049 by up-regulating a larger number of genes, most of them encoding proteins with regulatory and signaling functions. The data supports the idea that at least some of the phenotypic differences between slow wilting and drought sensitive plants may rely on the regulation of the level and timing of expression of specific genes. One of the genes that exhibited a marked N7001-specific drought induction profile encoded a eukaryotic translation initiation factor iso4G (GmeIFiso4G-1a). GmeIFiso4G-1a is one of four members of this protein family in soybean, all of them sharing high sequence identity with each other. In silico analysis of GmeIFiso4G-1 promoter sequences suggested a possible functional specialization between distinct family members, which can attain differences at the transcriptional level. Conditional overexpression of GmeIFiso4G-1a in Arabidopsis conferred the transgenic plants increased tolerance to osmotic, salt, drought and low temperature stress, providing a strong experimental evidence for a direct association between a protein of this class and general abiotic stress tolerance mechanisms. Moreover, the results of this work reinforce the importance of the control of protein synthesis as a central mechanism of stress adaptation and opens up for new strategies for improving crop performance under stress. © 2018 Gallino, Ruibal, Casaretto, Fleitas, Bonnecarrère, Borsani and Vidal.
653 $aABIOTIC STRESS
653 $aARABIDOPSIS
653 $aDROUGHT
653 $aEIFiso4G
653 $aSOYBEAN CROP
653 $aTRANSLATION INITIATION
700 1 $aRUIBAL, C.
700 1 $aCASARETTO, E.
700 1 $aFLEITAS, A.L.
700 1 $aBONNECARRERE, V.
700 1 $aBORSANI, O.
700 1 $aVIDAL, S.
773 $tFrontiers in Plant Science, 2018$gv.9, Article number 262. (2 March 2018). OPEN ACCESS
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Registro original : |
INIA Las Brujas (LB) |
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| Registros recuperados : 10 | |
| 2. |  | IRISARRI, P.; SOTELO, M.; CASARETTO, E.; BORSANI, O.; ZARZA, R.; REBUFFO, M.; MONZA, J. Diversidad genética de rizobios nativos aislados de nódulos de Lotus corniculatus en Uruguay. In: SIMPOSIO DE RECURSOS GENÉTICOS PARA AMERICA LATINA Y EL CARIBE, 7., 2009, Pucón, CL. Proceedings. [Temuco, CL: INIA], 2009. v. 2, p. 81-82.| Tipo: Trabajos en Congresos/Conferencias |
| Biblioteca(s): INIA La Estanzuela. |
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| 3. | | GALLINO, J.P.; RUIBAL, C.; CASARETTO, E.; FLEITAS, A.L.; BONNECARRERE, V.; BORSANI, O.; VIDAL, S. A dehydration-induced eukaryotic translation initiation factor iso4G identified in a slow wilting soybean cultivar enhances abiotic stress tolerance in Arabidopsis. Frontiers in Plant Science, 2018, v.9, Article number 262. (2 March 2018). OPEN ACCESS Article history: Received: 22 December 2017; Accepted: 14 February 2018; Published: 02 March 2018.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 4. | | SOTELO, M.; IRISARRI, P.; LORITE, M.J.; CASARETTO, E.; REBUFFO, M.; SANJUAN, J.; MONZA, J. Diversity of rhizobia nodulating Lotus corniculatus grown in northern and southern regions of Uruguay. Applied Soil Ecology,Volume 49, September 2011, Pages 197-207. Article history: Article history: Received 6 October 2010/Received in revised form 12 May 2011/Accepted 13 May 2011.| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : A - 1 |
| Biblioteca(s): INIA La Estanzuela. |
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| 5. | | BORSANI, O.; CASARETTO, E.; MÁRQUEZ, A.J.; REBUFFO, M.; DÍAZ, P.; MONZA, J. Metabolismo de prolina en cultivares de Trifolium pratense con diferente producción estival. In: BONETE, M.J.; MARTÍNEZ-ESPINOSA, R.M. (Coord.). Avances en el metabolismo del nitrógeno: de la genómica y la proteómica a las aplicaciones agronómicas, industriales y medioambientales. [San Vicente, Alicante], ES: Club Universitario, 2010. p. 319-325.| Tipo: Capítulo en Libro Técnico-Científico |
| Biblioteca(s): INIA La Estanzuela. |
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| 6. | | SIMONDI, S.; CASARETTO, E.; QUERO, S.; CERETTA, S.; BONNECARRERE, V.; BORSANI, O. A simple and accurate method based on a water-consumption model for phenotyping soybean genotypes under hydric deficit conditions. Agronomy, 2022, Volume 12, Issue 3, Article number 575. GOLD OPEN ACCESS. doi: https://doi.org/10.3390/agronomy12030575 Article history: Received 14 December 2021; Revised 17 February 2022; Accepted 24 February 2022; Published 25 February 2022.
Corresponding author: Borsani, O.; Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la...| Tipo: Artículos en Revistas Indexadas Internacionales | Circulación / Nivel : Internacional - -- |
| Biblioteca(s): INIA Las Brujas. |
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| 7. | | SOTELO, M.; IRISARRI, P.; LORITE, M.J.; CASARETTO, E.; BORSANI, O.; CUITIÑO, M.J.; REBUFFO, M.; SANJUAN, J.; MONZA, J. Characterization of the genetic diversity of rhizobia isolated from nodules of Lotus corniculatus and response to inoculation in Uruguay. Lotus Newsletter, v. 41, p. 7-9, 2011. En prensa.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA La Estanzuela. |
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| 8. | | VIDAL, S.; GALLINO, J.P.; RIVERO, Y.; RUIBAL, C.; FLEITAS, L.; CASTILLO, A.; CERETTA, S.; CASARETTO, E.; BORSANI, O. Biotecnología aplicada al mejoramiento genético de la soja. Montevideo (UY): INIA, 2018. 37 p. (Serie FPTA-INIA; 68) Proyecto FPTA-309: Biotecnología aplicada al mejoramiento genético de la soja. Período de ejecución: Mayo 2014-Noviembre 2017. Institución Ejecutora: Universidad de la República, Facultad de Ciencias. Responsable del Proyecto: Sabina Vidal.| Biblioteca(s): INIA Las Brujas. |
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| 9. | | BERGER, A.; BONNECARRERE, V.; BORSANI, O.; CASARETTO, E.; CASTILLO, A.; CASTRO, A.; CERETTA, S.; FLEITAS, L.; GALLINO, J.P.; GARAYCOCHEA, S.; IRIARTE, W.; KAVANOVÁ, M.; QUERO, G.; REY, E.; RUIBAL, C.; SIMONDI, S.; VIDAL, S. Aproximaciones genómicas, fisiológicas y de mejoramiento para incrementar la tolerancia a sequía en soja. In: INIA (Instituto Nacional de Investigación Agropecuaria); INIA Las Brujas; Biotecnología. Jornada de Agrobiotecnología, X. Encuentro Nacional de REDBIO, II. Jornada técnica. Las Brujas, Canelones (UY): INIA, 2017. (Serie Actividades de Difusión; 780)| Biblioteca(s): INIA Las Brujas. |
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| 10. | | REBUFFO, M.; ZARZA, R.; BORSANI, O.; CASARETTO, E.; MESSA, A.; SAINZ, M.M.; SALDIAS, R.; ALZUGARAY, R.; CONDON, F.; DIAZ, P.; MONZA, J.; RISSO, D.; BEMHAJA, M.; BERMÚDEZ, R.; AYALA, W.; ALTIER, N.; ZARZA, M. Genetic resources of forage legumes for agriculture-pastoral system in Uruguay. Lotus Newsletter, 2007, Volume 37, Issue 2, pages 56.| Tipo: Abstracts/Resúmenes |
| Biblioteca(s): INIA La Estanzuela. |
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| Registros recuperados : 10 | |
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